Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli.
To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its precursor ProROL in soluble form was achieved when E. coli Origami(DE3) and pET-11d were used as expression systems.
متن کاملOptimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
The lipases of the Rhizopus species family are important and versatile enzymes that are mainly used in fat and oil modification due to their strong 1,3-regiospecificity. Inexpensive synthetic medium was used for the production of Rhizopus oryzae lipase in the methylotrophic yeast Pichia pastoris. Methanol accumulation inside the bioreactor has previously been shown to negatively influence the p...
متن کاملA comparison on Lipase Production from Soybean meal and Sugarcane Bagasse in Solid State Fermentation using Rhizopus oryzae
In this study, solid-state fermentation of two types of agricultural residues/products for lipase production in a tray-bioreactor was investigated. Rhizopus oryzae was used as a potential fungus strain and two types of agricultural residues including soybean meal and sugarcane bagasse were utilized as substrate. Fermentation was carried out in two different operational conditions: one with cont...
متن کاملCombined Strategies for Improving the Production of Recombinant Rhizopus oryzae Lipase in Pichia pastoris
We have developed a yeast Pichia pastoris system for the high-level expression of recombinant Rhizopus oryzae lipase (ROL), which is a potentially effective catalyst in the solvent-free production of biodiesel fuel. In the glycerol fed-batch phase, the combination of the dissolvedoxygen-stat and gradient-control glycerol feeding strategies resulted in a higher cell biomass in the P. pastoris cu...
متن کاملModelling and optimization of ethyl butyrate production catalysed by Rhizopus oryzae lipase
Response surface methodology was used to model and optimise the production of ethyl butyrate, catalysed by Rhizopus oryzae lipase immobilised in a hydrophilic polyurethane foam. Experiments were carried out following a central composite rotatable design, as a function of reaction temperature (T: 22–38 C) initial butyric acid concentration (A: 0.031–0.619 M) and initial molar ratio ethanol/acid ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2005
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.71.12.8974-8977.2005